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Laboratory Profile
  1. Immunotherapy for Malignant Mesothelioma that Combines CXCL12/CXCR4 Blockade with a Mesothelin-Targeted Fusion Protein
  2. Immunotherapy for Mesothelioma and Ovarian Caner with an in vivo DC Vaccine and PD-1/PD-L1 Blockade
  3. Injectable Extracellular Matrix-Derivative Micro-Scaffolds (iEMS) mouse tumor model in drug evaluation and cancer immunotherapy
  4. Profiling the Immune Contexture in Human Cancers and Infectious Diseases
  • Principal investigator: Huabiao Chen, MD, PhD
  • Postdoctoral Research Fellows: Yang (Claire) Zeng, MD, PhD ; Xiying Qu, PhD
  • Research Scientist:
  • Peter Rees, BA candidate
  • Zhao Liu, MD, PhD
  • Alice Tang, Imperial College
  • Binghao Li, MD candidate
  • Qiuyan Liu, MD, PhD; Yingying Liang, MSc
Original Research Articles (* corresponding authors)
2018
  1. Binghao Li, Yang Zeng, Patrick M. Reeves, Chongzhao Ran, Qiuyan Liu, Xiying Qu, Yingying Liang, Zhao Liu, Jianping Yuan, Pierre R. Leblanc, Zhaoming Ye, Ann E. Sluder, Jeffrey A. Gelfand, Timothy A. Brauns, Huabiao Chen*, and Mark C. Poznansky. AMD3100 augments the efficacy of mesothelin-targeted immune-activating fusion protein in mesothelioma by multi-modal downmodulation of intratumoral immunosuppression. Cancer Immunol Res 2018;6:539-551.
  2. Binghao Li, Zhan Wang, Hao Wu, Mingfeng Xue, Peng Lin, Shengdong Wang, Nong Lin, Xin Huang, Weibo Pan, Meng Liu, Xiaobo Yan, Hao Qu, Lingling Sun, Hengyuan Li, Yan Wu, Wangsiyuan Teng, Zenan Wang, Xingzhi Zhou, Huabiao Chen, Mark Poznansky, and Zhaoming Ye. Epigenetic regulation of CXCL12 plays a critical role in mediating tumor progression and the immune response in osteosarcoma. Cancer Res 2018;78:3938-3953.
  3. Alok V. Joglekar, Zhe Liu, Jeffrey K. Weber, Yong Ouyang, Won Jun Noh, Pedro A. Lamothe-Molina, Huabiao Chen, Seung-gu Kang, Michael T. Bethune, Ruhong Zhou, Bruce D. Walker, David Baltimore. HIV-specific T cell receptors from patients with differential immunologic control are functionally indistinguishable. PNAS 2018; published ahead of print February 7, 2018, https://doi.org/10.1073/pnas.1718659115.
  4. Tao Li, Jun Zhang, Jiahui Zhang, Nannan Zhang, Yang Zeng, Shengnan Tang, Zehua Tao, Xiying Qu, Jue Jia, Wei Zhu, Xiaochun Sun and Huabiao Chen*. Nicotine-enhanced stemness and epithelial-mesenchymal transition of human umbilical cord mesenchymal stem cells promote tumor formation and growth in nude mice. Oncotarget. 2018; 9(1):591-606. https://doi.org/10.18632/oncotarget.22712
  5. Zhang J, Zhang J, Li T, Zhang N, Tang S, Tao Z, Zhou X, Sun X, Chen H*. Effect of idebenone on bone marrow mesenchymal stem cells in vitro. Mol Med Rep. 2018 Jan 26. doi: 10.3892/mmr.2018.8506. [Epub ahead of print]
  6. Sun W, Shi J, Wu J, Zhang J, Chen H, Li Y, Liu S, Wu Y, Tian Z, Cao X, Li N. A modified HLA-A*0201-restricted CTL epitope from human oncoprotein (hPEBP4) induces more efficient antitumor responses. Cell Mol Immunol. 2018 Jan 29. doi: 10.1038/cmi.2017.155. [Epub ahead of print]
  7. 2017
  8. Mattox A, Lee J, Westra WH, Pierce RH, Ghossein R, Faquin WC, Diefenbach TJ, Morris LG, Lin DT, Wirth LJ, Lefranc-Torres A, Ishida E, Chakravarty PD, Johnson L, Zeng Y, Chen H, Poznansky MC, Iyengar NM, Pai SI. PD-1 expression in head and neck squamous cell carcinomas derives primarily from functionally anergic CD4+ TILs in the presence of PD-L1+ TAMs. Cancer Res. 2017 Nov 15;77(22):6365-6374. doi: 10.1158/0008-5472.CAN-16-3453.
  9. Leitman EM, Willberg CB, Tsai MH, Chen H, Buus S, Chen F, Riddell L, Haas D, Fellay J, Goedert JJ, Piechocka-Trocha A, Walker BD, Martin J, Deeks S, Wolinsky SM, Martinson J, Martin M, Qi Y, Sáez-Cirión A, Yang OO, Matthews PC, Carrington M, Goulder PJR. HLA-B*14:02-Restricted Env-Specific CD8+ T-Cell Activity Has Highly Potent Antiviral Efficacy Associated with Immune Control of HIV Infection. J Virol. 2017 Oct 27;91(22). pii: e00544-17. doi: 10.1128/JVI.00544-17.
  10. Morse K, Kimizuka Y, Chan MPK, Shibata M, Shimaoka Y, Takeuchi S, Forbes B, Nirschl C, Li B, Zeng Y, Bronson RT, Katagiri W, Shigeta A, Sîrbulescu RF, Chen H, Tan RYY, Tsukada K, Brauns T, Gelfand J, Sluder A, Locascio JJ, Poznansky MC, Anandasabapathy N, Kashiwagi S. Near-Infrared 1064 nm Laser Modulates Migratory Dendritic Cells To Augment the Immune Response to Intradermal Influenza Vaccine. J Immunol. 2017 Aug 15;199(4):1319-1332.
  11. Ding Y, Zhang C, Zhang J, Zhang N, Li T, Fang J, Zhang Y, Zuo F, Tao Z, Tang S, Zhu W, Chen H*, Sun X. miR-145 inhibits proliferation and migration of breast cancer cells by directly or indirectly regulating TGF-β1 expression. Int J Oncol. 2017 May;50(5):1701-1710.
  12. Kimizuka Y, Callahan JJ, Huang Z, Morse K, Katagiri W, Shigeta A, Bronson R, Takeuchi S, Shimaoka Y, Chan MP, Zeng Y, Li B, Chen H, Tan RY, Dwyer C, Mulley T, Leblanc P, Goudie C, Gelfand J, Tsukada K, Brauns T, Poznansky MC, Bean D, Kashiwagi S. Semiconductor diode laser device adjuvanting intradermal vaccine. Vaccine. 2017 Apr 25;35(18):2404-2412.
  13. Lu Zhu, Xingliang Fan, Bingjie Wang, Longwei Liu, Xiaojun Yan, Lyu Zhou, Yang Zeng, Mark C. Poznansky, Lili Wang, Huabiao Chen, Yanan Du. Biomechanically primed liver microtumor array as a high-throughput mechanopharmacological screening platform for stroma-reprogrammed combinatorial therapy. Biomaterials. http://dx.doi.org/10.1016/j.biomaterials.2017.01.030
  14. Flerin NC, Chen H, Glover TD, Lamothe PA, Zheng JH, Fang JW, Ndhlovu ZM, Newell EW, Davis MM, Walker BD, Goldstein H. TCR Clonotype-specific Differences in Inhibitory Activity of HIV-1 Cytotoxic T Cell Clones is Not Mediated by TCR Alone. J Virol. 2017 Jan 11. pii: JVI.02412-16. doi: 10.1128/JVI.02412-16.
  15. 2016
  16. Yang Zeng, Binghao Li, Patrick Reeves, Chongzhao Ran, Zhao Liu, Mojgan Agha-Abbaslou, Jianping Yuan, Pierre Leblanc, Ann Sluder, Jeffrey Gelfand, Timothy Brauns, Mark Poznansky, Huabiao Chen*. Improved antitumoral efficacy of mesothelin targeted immune activating fusion protein in murine model of ovarian cancer. Int J Cancer Clin Res. 2016, 3: 051
  17. 2015
  18. Chunfu Zhang, Kui Liu, Tao Li, Jie Fang, Yanling Ding, Lingxian Sun, Tao Tu, Xinyi Jiang, Shanmei Du, Jiabo Hu, Wei Zhu, Huabiao Chen*, and Xiaochun Sun. miR-21: A gene of dual regulation in breast cancer. Int J Oncol. 2015 Nov 5. doi: 10.3892/ijo.2015.3232. [Epub ahead of print].
  19. Ndhlovu ZM, Stampouloglou E, Cesa K, Mavrothalassitis O, Alvino DM, Li JZ, Wilton S, Karel D, Piechocka-Trocha A, Chen H, Pereyra F, Walker BD. The Breadth of Expandable Memory CD8+ T Cells Inversely Correlates with Residual Viral Loads in HIV Elite Controllers. J Virol. 2015 Nov 1;89(21):10735-47. doi: 10.1128/JVI.01527-15. Epub 2015 Aug 12.
  20. Tao Li, Chunfu Zhang, Yanling Ding, Wei Zhai, Kui Liu, Fan Bo, Tao Tu, Lingxian Sun, Wei Zhu, Fangfang Zhou, Wenkai Qi, Jiabo Hu, Huabiao Chen*, and Xiaochun Sun. Umbilical cord-derived mesenchymal stem cells promote proliferation and migration in breast cancer cells MCF-7 and MDA-MB-231 through activation of the ERK pathway. Oncol Rep 2015; 34: 1469-1477.
  21. Kui Liu, Chunfu Zhang, Tao Li, Yanling Ding, Tao Tu, Fangfang Zhou, Wenkai Qi, Huabiao Chen* and Xiaochun Sun. Let-7a inhibits growth and migration of breast cancer cells by targeting HMGA1. Int J Oncol 2015;46:2526-2534.
  22. Dongfang Liu, Huabiao Chen*. Structured Illumination Microscopy Improves Visualization of Lytic Granules in HIV-1 Specific CTL Immunological Synapses. AIDS Res Hum Retroviruses 2015 Apr 1. [Epub ahead of print].
  23. 2014
  24. Wang P, Qu X, Wang X, Zhu X, Zeng H, Chen H, Zhu H. Specific reactivation of latent HIV-1 with designer zinc-finger transcription factors targeting the HIV-1 5'-LTR promoter. Gene Ther. 2014;21:490-495.
  25. Zhu X, Liu S, Wang P, Qu X, Wang X, Zeng H, Chen H, Zhu H. Oxaliplatin antagonizes HIV-1 latency by activating NF-κB without causing global T cell activation. Biochem Biophys Res Commun. 2014;450:202-207.
  26. Jianping Yuan, Satoshi Kashiwagi, Jean Nezivar, Yuan Yang, Mai Nguyen, Huabiao Chen, Sandra Orsulic, Nathalie Scholler, Glenn Dranoff, Timothy Brauns, Jeffrey Gelfand, Pierre Leblanc and Mark C. Poznansky. A Mycobacterial Hsp70 Fusion Protein Targeting Mesothelin Augments Antitumor Immunity and Prolongs Survival in Murine Models of Ovarian Cancer and Mesothelioma. J Hematol Oncol 2014;7:15-24.
  27. Maria H. Foley, Talitha Forcier, Elizabeth McAndrew, Michael Gonzalez, Huabiao Chen, Boris Juelg, Bruce D. Walker, and Darrell J. Irvine. High Avidity CD8(+) T Cells Efficiently Eliminate Motile HIV-Infected Targets and Execute a Locally Focused Program of Anti-Viral Function. Plos One 2014;9:e87873.
  28. 2013
  29. Zhen Xia, Huabiao Chen, Seung-gu Kang, Tien Huynh, Justin W. Fang, Pedro A. Lamothe, Bruce D. Walker, and Ruhong Zhou. The complex and specific pMHC interactions with diverse HIV-1 TCR clonotypes reveal a structural basis for alterations in CTL function. Sci Rep 2013; 4:4087.
  30. 2012
  31. Huabiao Chen, Zaza M. Ndhlovu, Dongfang Liu, Lindsay C. Porter, Sam Darko, Justin W. Fang, Mark A. Brockman, Toshiyuki Miura, Zabrina L. Brumme, Arne Schneidewind, Alicja Piechocka-Trocha, Kevin T. Cesa, Jennifer Sela, Thai D. Cung, Ildiko Toth, Florencia Pereyra, Xu G. Yu, Daniel C. Douek, Daniel E. Kaufmann, Todd M. Allen, Bruce D. Walker. TCR clonotypes modulates the protective effect of HLA class I molecules in HIV-1-infection. Nat Immunol 2012;13:691-700.
  32. Zhang SC, Martin E, Shimada M, Godfrey SB, Fricke J, Locastro S, Lai NY, Liebesny P, Carlson JM, Brumme CJ, Ogbechie OA, Chen H, Walker BD, Brumme ZL, Kavanagh DG, Le Gall S. Aminopeptidase substrate preference affects HIV epitope presentation and predicts immune escape patterns in HIV-infected individuals. J Immunol 2012;188(12):5924-5934.
  33. 2011
  34. Huabiao Chen, Chun Li, Jinghe Huang, Thai Cung, Jill Beamon, Katie Seiss, Mary Carrington, Lindsay C. Porter, Patrick Burke, Yue Yang, Bethany Ryan, Ruiwu Liu, Robert Weiss, Florencia Pereyra, William Cress, Abraham L. Brass, Eric S. Rosenberg, Bruce D. Walker, Xu G. Yu, Mathias Lichterfeld. CD4 T cells from elite controllers resist HIV-1 infection by selective upregulation of p21 (cip-1/waf-1). J Clin Invest 2011;121:1549-1560.
  35. Navin Varadarajan, Boris Julg, Yvonne Yamanaka, Huabiao Chen, Adebola Ogunniyi, Elizabeth McAndrew, Lindsay Porter, Alicja Piechocka-Trocha, Brenna Hill, Daniel Douek, Florencia Pereyra, Bruce D. Walker, J. Christopher Love. A high-throughput single-cell analysis of human CD8+ T cell functions reveals discordance for cytokine secretion and cytolysis. J Clin Invest, 2011;121:4322-4331.
  36. Before 2010
  37. Huabiao Chen, Alicja Piechocka-Trocha, Toshiyuki Miura, Mark A. Brockman, Boris D. Julg, Brett M. Baker, Alissa C. Rothchild, Brian L. Block, Arne Schneidewind, Tomohiko Koibuchi, Florencia Pereyra, Todd M. Allen, Bruce D. Walker. Differential neutralization of HIV replication in autologous CD4 T cells by HIV-specific CTL. J Virol 2009;83:3138-3149.
  38. Jianping Yuan, Ping Li, Jing Tao, Xiaodong Shi, Baoyu Hu, Huabiao Chen and Xiaokui Guo. H. pylori escape host immunoreaction through inhibiting ILK expression by VacA. Cell Mol Immunol 2009; 6:191-197.
  39. Arne Schneidewind, Mark A. Brockman, John Sidney, Yaoyu E. Wang, Huabiao Chen, Todd J. Suscovich, Bin Li, Rahma I. Adam, Rachel L. Allgaier, Bianca R. Mothé, Thomas Kuntzen, Cesar Oniangue-Ndza, Alicja Trocha, Xu G. Yu, Christian Brander, Alessandro Sette, Bruce D. Walker, and Todd M. Allen. Structural and functional constraints limit options for cytotoxic T-lymphocyte escape in the immunodominant HLA-B27-restricted epitope in human immunodeficiency virus type 1 capsid. J Virol 2008: 82:5594-605.
  40. Huiying Jin, Kaihua Tao, Huabiao Chen, Yuexi Li, Faqing Li. Preparation of DNA microchip for detection of vibrio cholerae and enterohemorrhagic E.coli O157:H7. Chin J Zoonoses 2006;22:443-446.
  41. Huabiao Chen, Jinlin Hou, Xiaodong Jiang, Shiwu Ma, Minjie Meng, Baomei Wang, Minghui Zhang, Mingxia Zhang, Xiaoping Tang, Fuchun Zhang, Tao Wan, Nan Li, Yizhi Yu, Hongbo Hu, Ruifu Yang, Wei He, Xiaoning Wang and Xuetao Cao. Response of memory CD8+ T cells to severe acute respiratory syndrome (SARS) coronavirus in recovered SARS patients and healthy individuals. J Immunol 2005;175:591-598.
  42. Baomei Wang, Huabiao Chen, Xiaodong Jiang, Minghui Zhang, Tao Wang, Nan Li, Xiangyang Zhou, yanfeng Wu, Feng Yang, Yizhi Yu, Xiaoning Wang, Ruifu Yang and Xuetao Cao. Identification of an HLA-A*0201-restricted CD8+ T-cell epitope SSp-1 of SARS-CoV spike protein. Blood 2004;104:200-206.
  43. Jianping Yuan, Tao Li, Huabiao Chen, Zhenhong Li, Guizhen Yang, Baoyu Hu, Xiaoding Shi, Shanqing Tong, Yixue Li and Xiaokui Guo. Analysis of gene expression profile in gastric cancer cells stimulated with Helicobacter pylori isogenic strains. J Med Microbiol 2004;53:965-974.
  44. Qiuyan Liu, Taoying Chen, Huabiao Chen, Minghui Zhang, Nan Li, Zhanjun Lu, Pengcheng Ma and Xuetao Cao. Triptolide (PG-490) induces apoptosis of dendritic cells through sequential p38 MAP kinase phosphorylation and caspase 3 activation. Biochem Biophys Res Commun 2004;319:980-986.
  45. Huabiao Chen*, Dexing Fang, Faqing Li, Huiying Jin, Weiguo Tan and Suqin Li. A novel hepatitis B virus mutant with A-to-G at nt551 in the surface antigen gene. World J Gastroenterol 2003;9:304-308.
  46. Chunling Ma, Dexing Fang, Huabiao Chen, Faqing Li, Huiying Jin, Suqin Li, Weiguo Tan. A mutation specific polymerase chain reaction of detecting hepatitis B virus genome mutations at nt551. World J Gastroenterol 2003;9:509-512.
  47. Faqing Li, Huiying Jin, Dexing Fang, Weiguo Tan, Suqin Li, and Huabiao Chen. Experimental study of Hepatitis A virusemia and in vitro multiplication. J Chin Hepatol 2003;6:11-13.
  48. Huiying Jin, Huabiao Chen, Kaihua Tao, Yuexi Li, Faqing Li, Suqin Li and Weiguo Tan. Detection of Enterohemorrhagic E. coli O157:H7 and vibrio cholerae by multiplex PCR assay. J Prev Med Chin PLA 2003;21:252-255.
  49. Huabiao Chen, Huiying Jin, Kaihua Tao, Faqing Li, and Yuexi Li. Detection of pathogenic comma bacillus by multi-PCR. Chin J Lab Med 2002;25:304.
  50. Faqing Li, Weiguo Tan, Suqin Li, Nan Yang, Huiying Jin, Huabiao Chen. Preclinical observation of drug safety of human nerve growth factor injection. Med J Nanjin Mil 2002;4:28-30.
  51. Huabiao Chen, Dexing Fang, Faqing Li, Huiying Jin, Weiguo Tan, Suqin Li. Purification and characterization of the hepatitis B surface antigen fragment with 124 amino acids expressed in E.coli. Jiangsu Med J 2001;27:898-900.
  52. Faqing Li, Huiying Jin, Weiguo Tan, Suqin Li, Xiuzhen Pan, Dexing Fang, and Huabiao Chen. Dynamic detection of hepatitis A virus antigen or antibody in sera and faeces of 19 patients with hepatitis A. J P Hepatol 1999;4:7-8.
  53. Dexing Fang, Faqing Li, Weiguo Tan, Yuanlun Wang, Huabiao Chen, Houji Lin, zhenxian Zhou. A mutation specific polymerase chain reaction for detecting hepatitis B virus genome with A to C mutation at nt585. Chin J Exp Clin Virol 1999;13:373-376.
  54. Dexing Fang, Houji Lin, Faqing Li, Zhenxian Zhou, Weiguo Tan, Yuanlun Wang, and Huabiao Chen. Nucleotide sequencing of the S gene of a new hepatitis B virus immune escape mutant. Chin J Microbiol Immunol 1999;19:326-329.
  55. Suqin Li, Faqing Li, Weiguo Tan, Nan Yang, Huiying Jin, Huabiao Chen. An improved, inexpensive method for the large-scale purification of human nerve growth factor. Biotechnol Appl Biochem 1998;28:215-218.
  56. Huabiao Chen, Shufang Jiang. Detection of IgM antiidiotype antibodies against anti-HCV (IgM anti-HCV-Ab2) by ELISA. Chin J Immunol 1997;13:124-126.
  57. Huabiao Chen, Xiaozhao Deng, Guangfu Li, Zhengyu Diao, Linyuan Zhang. Purification and characterization of recombinant human interferon α2b. Pharmaceutical Biotechnology 1997;4:9-12.
  58. Guangfu Li, Xiaozhao Deng, Linyuan Zhang, Zhengyu Diao, Huabiao Chen, and Jianhua Chai. Construction of human interferon α1 gene eukaryotic expression vector and its activity determination. Pharmaceutical Biotechnology 1996;3:78-81.
  59. Xiaozhao Deng, Yong Diao, Zhengyu Diao, Guangfu Li, Huabiao Chen, Linyuan Zhang. Purification and characterization of recombinant human interferon α1 expressed in eukaryotic expression vector rBmNPV. Pharmaceutical Biotechnology 1996;3:73-77.
  60. Guangyong Peng, Shufang Jiang, Huabiao Chen. Detection of HCV-IgM complex by ELISA in patients with hepatitis C. Shanghai J Med Lab Sci 1995;10:62.